Fig. 2
From: Discovery of effectors for casein kinase signaling in the African trypanosome
BBP59, LRRP1, BBP268, and CAP64 are phosphorylated by CK1.2 in vitro. (A) Peptides from CK1.2-pathway proteins. Six residues flanking predicted phosphosites (in red) were included in synthesized peptides. (B) Peptide phosphorylation by CK1.2. Peptides were tested as sets in three independent assays. Each peptide was tested in two technical replicates per (independent) experiment. Symbols represent mean counts per minute (CPM) of the technical replicates and are color and shape-coded according to the experimental replicate. Blue circles were measurements from the first experiment, red squares from the second, and black triangles from the third. Lines represent mean CPM values. A repeated measures one-way ANOVA with Dunnett’s multiple comparisons test was used to test for significance between each peptide and a “No Substrate” control. BBP59, p = 0.0001; LRRP1, p = 0.0077; Tb11.01.2730, p = 0.9996; FAZ2, p = 0.0548; BBP268, p < 0.0001, CAP64, p = 3.3 × 10–4.
