Fig. 6
From: Discovery of effectors for casein kinase signaling in the African trypanosome
Phosphomimetic residues at Ser72 alter BBP59 effects on kDNA division and cytokinesis. T. brucei expressing of ectopic BBP59 was induced for 4 h with tetracycline. Kinetoplasts (K) and nuclei (N) were stained with DAPI, visualized by fluorescence microscopy and enumerated. Statistical significance of the differences in cell types between parental single marker (SM) controls and induced trypanosomes expressing variants of BBP59 was determined with a χ2 test. (A) S72_BBP59 (unmutated) and SM T. brucei (p = 1.4 × 10–10). (B) A72_BBP59 (p = 3.3 × 10–8) and D72_BBP59 (p = 4.2 × 10–4) were compared to unmutated S72_BBP59. (C) Plots of KinD:Cyto ratios in cells with different genetic backgrounds. Cartoon shows major effects of D72_BBP59 and A72_BBP59 on cytokinesis and kDNA division. (D) Model (inferred from panel C) of effect of Ser72 phosphorylation on BBP59 regulation of cytokinesis and kDNA division.
