Fig. 7
From: Discovery of effectors for casein kinase signaling in the African trypanosome
Expression of A72_BBP59 changes the phosphoproteome of T. brucei. In SILAC-based phosphoproteomics (three independent experiments) A72_BBP59 expression was induced with tetracycline for 4 h in trypanosomes cultured in heavy SILAC medium. Un-induced A72_BBP59 cells were grown in light SILAC medium as a control. Phosphopeptides were enriched and identified using LC-FAIMS-MS/MS and a 1% False Discovery Rate. Phosphopeptides observed in at least two independent biological samples replicates with a two-fold change (at least) in abundance between light and heavy samples were accepted as positive results. Representative phosphopeptides are presented by plotting relative abundance against Qvality posterior error probability (PEP) score. Highlighted phosphopeptides (red) belong to proteins essential for cytokinesis.
