Fig. 2
FLASH irradiation preserves cell viability and metabolic activity in ARPE-19 cells. (A) MTT assay of ARPE-19 culture plates after exposures to 4, 8 and 16 Gy irradiation, in FLASH (red columns) and CONV (blue columns) modalities. Controls (gray columns) are age-matched culture plates, non-exposed to irradiation. Readings are done 72 h after exposure. Results of MTT assay were pooled from 12 replicate samples derived from 2 independent experiments and expressed as mean ± S.D. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. p values are indicated in the graphs. (B) Bar graphs illustrating the survival rate of ARPE-19 cells 72 h post irradiation of increasing intensities, in FLASH (red columns) or CONV (blue columns) modalities. Controls (gray columns) are age-matched culture plates, non-exposed to irradiation. The experiment was conducted on 3 biological replicates for each condition. Data are expressed as mean ± SD. Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. p values are indicated in the graphs. (C) ARPE-19 cells stained with Ab against ZO-1 (red signal) and α-Tubulin (green signal). Blue signal is referred to Hoechst nuclear staining. (D) Representative image of ARPE-19 cells stained with antibodies against ZO-1 (red signal) and α-Tubulin (green signal) at higher magnification. Blue signal is referred to Hoechst nuclear staining. (E) Representative ARPE-19 cell images for each condition of irradiation at 4 Gy labeled with Hoechst nuclear staining, with arrows pointing to pyknotic nuclei.
