Fig. 5
Mapping of oxytocin and dopamine receptor expression across cell types in the prairie vole nucleus accumbens. (A) Oxtr and Drd transcript expression levels from prairie vole nucleus accumbens. Drd1 and Drd2 transcripts are expressed at high levels, whereas Drd3, Drd4, Drd5, and Oxtr transcript levels are significantly less abundant. (B) Single-nucleus RNA sequencing data from prairie voles identifying 15 molecularly-defined cell types in the nucleus accumbens. (C, D) Visualization of Oxtr and Drd transcript expression across all nucleus accumbens cell types (UMAP and violin plots). Single-nucleus RNA sequencing results support our FISH results in that Oxtr is expressed in both Drd1 and Drd2 cells, as well as in some astrocytes, mature oligodendrocytes, and all interneurons populations identified (somatostatin/neuropeptide Y, parvalbumin and GABAergic interneurons). Drd1 and Drd2 transcripts are expressed in medium spiny neuron populations (MSN’s) as well as astrocytes, oligodendrocytes, microglia, and various interneuron populations. Drd5 transcripts appear selectively enriched in GABAergic and cholinergic interneurons, whereas Drd3 transcripts are expressed across several cell types including MSN’s and sparsely across any other cell types.
