Fig. 3 | Scientific Reports

Fig. 3

From: SIRT1 antisense long noncoding RNA attenuates interleukin-1β-induced osteoarthritic gene expression in human chondrocytes through its mRNA interaction

Fig. 3

The effects of SIRT1 AS lncRNA inhibition. (A) Real-time PCR for sirtuin 1 antisense long noncoding RNA (SIRT1 AS lncRNA). The values are relative to the control expression level (n = 3). (B) Western blotting for SIRT1. Representative data are demonstrated from repeated experiments. The relative expression of SIRT1 is demonstrated in the graph (n = 5). (C) Real-time PCR for SIRT1, SRY-related HMG box-containing protein 9 (SOX9), aggrecan, matrix metalloproteinase 13 (MMP-13), and a disintegrin and metalloproteinase with thrombospondin motif 5 (ADAMTS-5) mRNA (n = 5). The values are relative to the control expression level. Data are represented as the dot plot of the individual data and median (n = 5); *p < 0.05, **p < 0.01. Full Western blotting is shown in Supplementary Fig. 3.

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