Fig. 5 | Scientific Reports

Fig. 5

From: SIRT1 antisense long noncoding RNA attenuates interleukin-1β-induced osteoarthritic gene expression in human chondrocytes through its mRNA interaction

Fig. 5

Ribonuclease protection assay (RPA) and stability assay for samples from normal human articular chondrocytes with knee (NHAC-kn). (A) Non-overlapping (P1) and overlapping (P2) primer positions for RPA. (B) Real-time PCR for the products from P1 and P2 primers with and without RNAse. The values are relative to the control expression level. Data are represented as the dot plot of the individual data and median (n = 5); **p < 0.01, ***p < 0.001. (C) RNA stability assay of SIRT1 mRNA expression using samples transfected with control plasmid or SIRT1 AS lncRNA. The values are relative to the control expression level. Data are represented as the mean ± standard deviation (SD) (n = 4); *p < 0.05.

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