Fig. 2
Analysis of vascular structure in a 3D human vascular culture system with SARS-CoV-2 infection and LPA treatment. (A, B) Representative image data of CD31-immunostained 3D-cultured HBMEC infected with SARS-CoV-2 Wuhan strain and the LPA-treated group. Pictures were taken from the bottom of the culture dish to 120 μm. The bar graph shows the measured volume of the 3D-cultured endothelial cell tube structure within a microscopic area. (C, D) 3D-cultured HRGEC infected with SARS-CoV-2 Wuhan strain and the LPA-treated group. (E, F) 3D-cultured HBMEC infected with Delta variant of SARS-CoV-2. The bar graph shows the measured volume of CD31 fluorescently labeled endothelial cell tube structures contained in the same amount of gel. All data are expressed as mean ± SD, n = 4 per group.**P < 0.01. ns, not significant. Scale bar, 100 μm.
