Fig. 5
Evaluation of therapeutic efficacy of LPA by transcriptome analysis of lung tissue from hamsters challenged with SARS-CoV-2. (A) Dot plot showing DEGs in lung tissues from control vs. SARS-CoV-2-infected hamsters. RNA-seq of lung tissue harvested 5 d after SARS-CoV-2 infection reveals the upregulation of multiple genes (red dots), including several pro-inflammatory cytokines, while LPA administration results in the downregulation of a subset of pro-inflammatory genes (blue dots). (B) Heatmap showing gene expression patterns across experimental groups. Pro-inflammatory genes that were upregulated by SARS-CoV-2 infection and suppressed by LPA treatment are highlighted in red boxes. (C) Gene Ontology analysis visualization showing the activation of significantly enriched inflammatory pathways in lung tissue, comparing mock-infected controls, SARS-CoV-2-infected hamsters, and SARS-CoV-2-infected hamsters treated with LPA. Key inflammatory pathways affected by LPA treatment are highlighted. Permission has been obtained from Kanehisa laboratories for using KEGG pathway database42.
