Fig. 5 | Scientific Reports

Fig. 5

From: Examination of an iPSC model of human eye development reveals progressive emergence of critical embryonic cell types

Fig. 5

Single cell analyses of SEAM cultures at Day 14. (a) UMAP plot visualization of ocular cell types identified 12 transcriptionally distinct clusters. (b) Clusters represent nascent and mature cell populations that correspond to presumptive zones observed in the SEAM cultures at Day 14. (c) Feature plots highlight expression of well-defined markers indicative of cell type-specific differentiation. (d) Concurrent expression of several genes indicative of a ciliary marginal zone (CMZ) cell identity support the presence of a CMZ-like cell population in Clusters 2 (‘Early optic cup’) and 4 (‘Late optic cup’) at Day 14. (e) Heatmap showing the top 15 significant DE transcription factor-related genes in each cluster (adjusted p-value < 0.05, LFC ≥ 1.2). Significant DE transcription factors were not detected for ‘Developing lens’ (Cluster 11). Red and blue indicate standard deviation above and below the mean, respectively.

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