Fig. 7 | Scientific Reports

Fig. 7

From: Examination of an iPSC model of human eye development reveals progressive emergence of critical embryonic cell types

Fig. 7

Single cell analyses of SEAM cultures at Day 28. (a) UMAP plot visualization of ocular cell types identified 14 transcriptionally distinct clusters. (b) Groups of clusters represent cell type-specific populations expected within each of the fully developed zones observed at Day 28. (c) Feature plots highlight expression of well-defined markers indicative of cell type-specific differentiation. (d) Concurrent expression of PAX6, TP63, CXCL14 and TXNIP highlight a putative population of limbal epithelial stem cells (LESC) predominantly enriched in Cluster 7 (‘Limbal epithelial stem cells’) at Day 28. (e) Heatmap showing the top 15 significant DE transcription factor-related genes in each cluster (adjusted p-value < 0.05, LFC ≥ 1.2). Red and blue indicate standard deviation above and below the mean, respectively.

Back to article page