Fig. 9 | Scientific Reports

Fig. 9

From: Examination of an iPSC model of human eye development reveals progressive emergence of critical embryonic cell types

Fig. 9

Temporal modeling of ocular differentiation in SEAMs using integrated data across timepoints. (a) Distribution of cells originally from Day 14 (pink) and Day 28 (light blue) after merging single cell data from both time points to generate a new UMAP describing dynamic changes in cell differentiation potential. (b) Pseudotime analysis of merged data reveals two distinct differentiation trajectories from least differentiated (blue; root cell indicated by red asterisk) to most differentiated (yellow). (c) Merged UMAP features 10 transcriptionally distinct clusters. RNA velocity vectors convey predicted differentiation directionality originating in Cluster 5 (‘General proliferating progenitors’) toward retinal derivatives (Cluster 4, ‘Mature retina’ and Cluster 2, ‘Late optic cup’) and anterior segment derivatives (Cluster 3 ‘Corneal epithelial cells/lens’ and Cluster 9 ‘Endothelial cells/pericytes’).

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