Fig. 4

Percentage of splicing inclusion (PSI) values of the spliced exons for (a) SRGAP3 in stable elevated CO₂ and fluctuating elevated CO₂, (b) genes in stable elevated CO₂ alone, (c) genes in fluctuating elevated CO₂ alone. SE: skipped exon; A3SS: alternative 3’ splice site; A5SS: alternative 5’ splice site; MXE: mutually exclusive exon; RI: retained intron. For SE and RI events, the PSI value refers to the inclusion level of the cassette exon or retained intron, respectively. For A3SS and A5SS, the PSI value refers to the inclusion of the longer exon. For MXE events, there are two sets of box plots with the first set referring to exon 1 PSI values and the second set referring to exon 2 PSI values. Alongside each gene is a schematic representation of the isoform structures from the differential splicing events. The blue rectangles represent the exons undergoing differential splicing, the red rectangles represent the exons adjacent to the spliced exon, and the connecting lines represent the introns. The purple rectangles represent the protein domains (Prosite and Pfam) overlapping the exons which was inferred from the Ensembl database. The longer green rectangles represent full-length transcript isoforms while the shorter green bar represents transcript isoforms with a premature stop codon. The unfilled rectangles represent 5’/3’ UTR regions. The arrows indicate gene orientation on DNA strands (positive or negative).