Fig. 5

Effects of adenosine signaling blockade on effector T cells. Expression of A2AR in T cell subsets in PBMCs and SUPT1 cells. Levels of A2AR RNA expression (nTPM) were compared, using the HTA datasets (n = 5–6) and the leukemia cell line data from the Human Protein Atlas. (A). Effects of adenosine signaling blockade on SUPT1 cell activation. SUPT1 cells were activated by anti-CD3/CD28 antibodies and co-cultured with PEO1 or PEO4 cells for 48 h in the presence of 2 and 10 µM CGS15943. SUPT1 cells were collected, stained, and analyzed by a flow cytometer to determine the percentage of the CD4+/CD8+CD25+ population (B). The levels of CD39 and CD73 expression in PEO1 and PEO4 cells. Total RNA was analyzed by quantitative RT-PCR for the levels of CD39 and CD73 mRNA. Data are means ± SD (n = 3) from three independent experiments. The levels of CD73 protein in PEO1, PEO4, and SUPT1 cells. Total protein was analyzed by western blotting (C).