Fig. 4
SAR induction in the D3-mediated signal deficient mutant. The d3 and wild type Shiokari plants were treated with BIT (0.5 mg in pot) using the soil-drenching method 3 days prior to gene expression analysis and challenge inoculation with rice blast fungus. (a) Expression of SAR marker gene. The expression levels of OsWRKY45 were normalized against the expression level of OsUBQ measured in the same samples. (b) Rice blast inoculation assay. Spreading lesions on the fourth leaf of rice plants were counted 5 days after inoculation. (c) Photograph of representative disease symptoms taken 5 days after inoculation. Different letters indicate significant differences between samples (ANOVA, Tukey’s test, p < 0.05).
