Fig. 2 | Scientific Reports

Fig. 2

From: First detection and entomological characterisation of invasive malaria vector Anopheles stephensi in sympatry with other vectors in Gayi Southern Niger Republic

Fig. 2

Agarose gel electrophoretic image showing the results of amplification of fragments of COXI and ITS2 from Anopheles species. (a). COXI gel showing a diagnostic band at 710 bp. Lanes 1 and 17: Hyperladder 100 DNA ladder [Bioline 100–1013 bp]; Lane 2 and 16: Negative controls; Lane 3–10: An. stephensi; Lane 11, 12, 13, 14 and 145 An. coluzzii, An. arabiensis, and An. gambiae s.s., Culex, and Ae. aegypti, respectively. (b). ITS2 gel showing An. stephensi diagnostic band at 438 bp (lanes 2–9) from simplex PCR with primers st-F and UD2-R only; Lane 1: Hyperladder 100 DNA ladder [Bioline 100–1013 bp]; lanes 11 and 25: Hyperladder 1 kb [Bioline 200–10037]; lane 10: Negative control; lanes 12–24: gel showing variable control bands (> 600 bp) using st-F, U5.8 S-F, and UD2-R primers which targets a fragment common to arthropods; lanes 12–19: An. stephensi amplicons; lanes 20–24: An. coluzzii, An. arabiensis, and An. gambiae s.s., Culex and Ae. aegypti.

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