Fig. 3

SREBP-2 overexpression promotes NSCLC cell proliferation, migration and invasion. PC-9 cells were infected with lentiviral vectors to establish mock or SREBP-2-overexpressed stable cell lines. (A) Expression levels of SREBP-2 in SREBP-2-overexpressed PC-9 stable cells were measured using Western blot analysis. (B) Relative mRNA levels of SREBP-2, HMGCS1, HMGCR and LDLR in SREBP-2-overexpressed PC-9 stable cells were examined by qRT-PCR. GAPDH served as an internal control. (C) SREBP-2-overexpressed PC-9 stable cells were treated with increasing concentrations of fatostatin (0, 10, 20, and 40 µM) for 72 hours. Cell proliferation rate of the treated PC-9 stable cells was tested by crystal violet staining method. (D) Cell migratory and invasive abilities of the treated PC-9 stable cells were examined using the Transwell method. Data are presented as the mean ± SD of at least 3 independent experiments. ** p < 0.01, *** p < 0.001, compared with the mock group. ^## p < 0.01, ^### p < 0.001, compared with the control group.