Table 3 Primer sequences and expected sizes of the PCR products.

From: Exploring virulence factors, virulome, and multidrug resistance of Klebsiella pneumoniae strains isolated from patients with central Line-associated bloodstream infections

Virulence genes

Primer sequence (5′ → 3′)

Expected size (bp)

References

EAST-1

(F) ATGCCATCAACACAGTATAT

(R) GCGAGTGACGGCTTTGTAGT

110

19

CNF-1

(F)AAGATGGAGTTTCCTATGCAGGAG

(R)CATTCAGAGTCCTGCCCTCATTATT

498

19

FimH

(F) TGCAGAACGGATAAGCCGTGG

(R) GCAGTCACCTGCCCTCCGGTA

508

20

rmpA

(F) ACTGGGCTACCTCTGCTTCA

(R) CTTGCATGAGCCATCTTTCA

535

12

iutA

(F) GGCTGGACATCATGGGAACTGG

(R) CGTCGGGAACGGGTAGAATCG

300

21

FyuA

(F) TGATTAACCCCGCGACGGGAA

(R) CGCAGTAGGCACGATGTTGTA

880

20

bla SHV−1

(F) GGCCGCGTAGGCATGATAGA

(R) CCCGGCGATTTGCTGATTTC

714

22

bla TEM−1

(F) CAGCGGTAAGATCCTTGAGA

(R) ACTCCCCGTCGTGTAGATAA

643

22

bla KPC

(F) CGTCTAGTTCTGCTGTCTTG

(R) CTTGTCATCCTTGTTAGGCG

798

23

bla NDM

(F) GGTTTGGCGATCTGGTTTTC

(R) CGGAATGGCTCATCACGATC

621

23

  1. F: Forward R: Reverse bp: Base pair.
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