Table 4 PCR thermocycling conditions.
Gene | Initial Denaturation °C/Time | Denaturation °C/Time | Annealing °C/Time | Extension °C/Time | Final Extension °C/Time | Number of Cycles | References |
|---|---|---|---|---|---|---|---|
EAST-1 | 95 °C/4 min | 94 °C/50s | 58 °C/1.5 min | 72 °C/1.5 min | 72 °C/10 min | 35 | |
CNF-1 | 95 °C/4 min | 94 °C/50s | 58 °C/1 min | 72 °C/45s | 72 °C/8 min | 30 | |
FimH | 95 °C/4 min | 94 °C/1 min | 56 °C/45s | 72 °C/1 min | 72 °C/10 min | 34 | |
rmpA | 94 °C/4 min | 94 °C/30s | 50 °C/40s | 72 °C/1 min | 72 °C/10 min | 30 | |
iutA | 94 °C/4 min | 94 °C/30s | 63 °C/40s | 72 °C/1 min | 72 °C/10 min | 30 | |
FyuA | 95 °C/4 min | 95 °C/50s | 58 °C/1 min | 72 °C/45s | 72 °C/8 min | 30 | |
bla SHV−1 | 95 °C/5 min | 94 °C/30s | 55 °C/1 min | 72 °C/45s | 72 °C/7 min | 30 | |
bla TEM−1 | 95 °C/5 min | 94 °C/30s | 52 °C/45s | 72 °C/45s | 72 °C/7 min | 30 | |
bla KPC | 95 °C/15 min | 94 °C/30s | 58 °C/90s | 72 °C/90s | 72 °C/10 min | 35 | |
bla NDM | 95 °C/15 min | 94 °C/30s | 58 °C/90s | 72 °C/90s | 72 °C/10 min | 35 |
