Fig. 6

PI3K/Akt-related pathway protein analysis in the DMEM(LG) and DMEM(HG) groups. In passage 4 cells, the levels of p-PI3K, p-Akt (Ser473), p-GSK3β (Ser9), p-mTOR, active β-catenin, Bcl-2, Cdk4, CXCR-4 and Cyclin D1 were significantly increased in the DMEM(LG) group, and the level of Bax was significantly decreased in the DMEM(LG) group. Relative levels were calculated as the relative change from the DMEM(HG) group. The test was performed on the cells from all the six donors. The experiment was performed in triplicate, and the mean value of every sample was used for group comparisons. Group comparisons were performed using the paired t-test. n = 6.