Fig. 2
From: M464 inhibits activation of NLRP3 inflammasome and inflammatory response in mice
M464 inhibits the expression of marker proteins associated with NLRP3 inflammasome activation in cells. Western Blotting was used to evaluate the expression of IL-1β and Caspase-1 in J774A.1 (A–D) and THP-1 (E–H) cells’ supernatants and lysates. ImageJ was used to quantitatively analyze the levels of mature IL-1β and Caspase-1 protein in the cell supernatant. (I) The impact of M464 on IL-1β secretion activated by the NLRP3 inflammasome in J774A.1 cell supernatant was evaluated. The experiment was conducted independently three times, and the results were presented as the mean ± SD. *P < 0.05, **P < 0.01.
