Fig. 5
From: M464 inhibits activation of NLRP3 inflammasome and inflammatory response in mice
M464 mitigates LPS/D-GalN-induced acute liver injury in mice. Following intragastric administration of M464 (15, 30, and 60 mg/kg) for 1 h, Mice were intraperitoneally injected with LPS at a dose of 50 μg/kg and D-GalN at 800 mg/kg and then sacrificed after 6 h of stimulation. The groups included Con (control), Mod (LPS injection), DXM (Dexamethasone treatment at 10 mg/kg), and M464 at different doses (15, 30, 60 mg/kg). (A) Gross examination of mice liver. (B) Liver-to-body weight ratio of mice. (C) HE staining to analyze histopathological changes in the liver of mice. Red arrows: Areas of hepatocyte hemorrhage; Black arrows: Areas of hepatocyte necrosis. Scale bars = 200 μm. (D) Histological scoring of the liver. (E, F) Serum levels of ALT and AST in mice. (G, H) Detection of SOD and MDA in serum, indicative of oxidative stress. (I) Detection of liver injury indicator IL-1β in mice serum. (J, K) Detection of mature IL-1β and Caspase-1 protein levels in liver tissues using Western Blotting. (L, M) Measurement of CASP1 and IL1B mRNA expression levels in mice liver tissue by RT-qPCR. (N) Analysis of ASC protein expression in liver tissues through immunohistochemical staining. Scale bars = 400 μm. Experimental results were expressed as the mean ± SD; n = 5, with *P < 0.05 and **P < 0.01.
