Fig. 6 | Scientific Reports

Fig. 6

From: Genetic analysis of a novel TSC1 splice mutation causing tuberous sclerosis without neurological phenotypes

Fig. 6

(a) The growth of wild-type and mutant cells for Minigene after transient transfection for 24 h. (b) After collecting the wild-type and mutant cells for precipitation, RNA was extracted and reverse-transcribed. The target gene was then amplified by PCR. (c) The amplified product was recovered and sequenced. After the c.363 + 668G > C mutation occurred, a 92 bp intron sequence was inserted between c.363 and c.364.

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