Fig. 5 | Scientific Reports

Fig. 5

From: Hyperbaric oxygen treatment attenuates naloxone-precipitated opioid withdrawal behaviors and alters microglial activity in male and female mice

Fig. 5

HBOT is associated with decreased microglial soma volume in the PVT of morphine-naloxone but not saline-naloxone treated male and female mice. (A) HBOT had no significant effect on microglial soma volume in saline-naloxone treated male mice (HBOT–: n = 3 mice/43 cells; HBOT+: n = 4 mice/61 cells). (B) In contrast, HBOT significantly reduced microglial soma volume in morphine-naloxone treated male mice (HBOT–: n = 4 mice/90 cells; HBOT+: n = 4 mice/57 cells). (C) Similarly, no significant effect of HBOT was observed in saline-naloxone treated female mice (HBOT–: n = 4 mice/85 cells; HBOT+: n = 4 mice/58 cells). (D) However, HBOT significantly reduced microglial soma volume in morphine-naloxone treated female mice (HBOT–: n = 3 mice/41 cells; HBOT+: n = 4 mice/71 cells). Data are presented as relative frequency histograms. *p < 0.05. Symbols: White triangles, saline-naloxone treated HBOT– mice; purple triangles, saline-naloxone treated HBOT + mice; white circles, morphine-naloxone treated HBOT– mice; purple circles, morphine-naloxone treated HBOT + mice.

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