Table 3 Verification of SAA variant origins of peptides detected by LC-MS/MS, cases 2–5.

From: Polymorphisms in SAA alter intrarenal amyloid distribution of AA amyloidosis in cats

Variant

Position

Sequence

Case 2

Case 3

Case 4

Case 5

G

OM

G

OM

P

G

P

G

v1, v3, v4

25–33

EANYIGADK

53

46

81

49

46

53

52

49

v2

25–29

EANYK

-

-

-

-

-

-

-

-

v1, v4

39–45

GNYDAAR

-

35

-

-

-

-

-

-

v2

39–46

GNYEAAQR

        

v3

39–46

GNYDAAQR

44

-

51

38

57

41

41

38

v4

46–55

RGPGGVWAAK

-

66

-

-

-

-

-

-

v1, v3

47–55

GPGGAWAAK

61

58

44

60

64

64

53

64

v2

49–55

GGAWAAK

-

-

-

-

-

-

-

-

Dominant SAA variant

v3

v4

v3

v3

v3

v3

v3

v3

  1. Amyloid deposits from various sites (G, glomeruli; OM, outer medulla; P, papilla) were analyzed. Each number indicates the peptide score; In Mascot, each peptide’s displayed score represents the highest-scoring among all spectra matching the same peptide sequence, representing the confident identification. At the bottom, the amyloidogenic SAA variant identified based on the recovered fragments is shown.
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