Fig. 4
From: Deletion of PTHrP nuclear localization sequence and carboxyl terminus leads to lung dysplasia
Deletion of PTHrP NLS and C-terminus caused activation of TGF-β/Smad signaling. A. Representative TGF-β1 immunohistochemical micrographs (400× magnification, scale bar = 25 μm). B. Representative Smad2 immunohistochemical micrographs (400× magnification, scale bar = 25 μm). C. Statistical results of TGF-β1 IHC-positive area percentage in WT and KI mice. D. Statistical results of Smad2 IHC-positive area percentage in WT and KI mice. E. Western blot results of TGF-β1 and Smad2 proteins in lung tissues, with β-actin serving as the loading control. F. Statistical comparison of TGF-β1 and Smad2 protein expression levels. (A-F) Data are from three independent biological replicates (n = 3 WT and 3 KI mice) Adjusted P-value = Original P-value × Number of multiple comparisons (Bonferroni correction) adjusted p = raw p × 4 *p < 0.05, **p < 0.01, ***p < 0.001.
