Fig. 5
From: Deletion of PTHrP nuclear localization sequence and carboxyl terminus leads to lung dysplasia
Deletion of PTHrP NLS and C-terminus reduced lung cell proliferation and promoted apoptosis and senescence. (A) Representative PCNA immunohistochemical micrographs (400× magnification, scale bar = 25 μm). (B) Representative cleaved Caspase-3 immunohistochemical micrographs (400× magnification, scale bar = 25 μm). (C) Statistical results of the percentage of PCNA-positive area in WT and KI mice. (D) Statistical results of the percentage of cleaved Caspase-3-positive area in WT and KI mice. (E) Western blot results of PCNA, Caspase-3, Bax, Bcl-2, p16, p19, p21, p27, and p53 proteins in lung tissues, with β-actin serving as the loading control. (F) Statistical comparison of PCNA, Caspase-3, Bax, Bcl-2, p16, p19, p21, p27, and p53 protein expression levels. (A-F) Data are from three independent biological replicates (n = 3 WT and 3 KI mice). Adjusted P-value = Original P-value × Number of multiple comparisons (Bonferroni correction) adjusted p = raw p ×11 *p < 0.05, **p < 0.01, ***p < 0.001.
