Fig. 6
From: Deletion of PTHrP nuclear localization sequence and carboxyl terminus leads to lung dysplasia
Deletion of PTHrP NLS and C-terminus promoted production of inflammatory cytokines in the lungs. (A) Representative IL-1β immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (B) Representative IL-6 immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (C) Representative TNF-α immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (D) Statistical results of the percentage of IL-1β-positive area in WT and KI mice. (E) Statistical results of the percentage of IL-6-positive area in WT and KI mice. (F) Statistical results of the percentage of TNF-α-positive area in WT and KI mice. (G) Western blot results of IL-1β, IL-6, TNF-α, and p65 proteins in lung tissues, with β-actin serving as the loading control. (H) Statistical comparison of IL-1β, IL-6, TNF-α, and p65 protein expression levels. (A-H) Data are from three independent biological replicates (n = 3 WT and 3 KI mice). Adjusted P-value = Original P-value × Number of multiple comparisons (Bonferroni correction) adjusted p = raw p ×7 *p < 0.05, **p < 0.01, ***p < 0.001.
