Fig. 8
From: Deletion of PTHrP nuclear localization sequence and carboxyl terminus leads to lung dysplasia
Deletion of PTHrP NLS and C-terminus promoted lung DNA damage. (A) Representative 8-OHdG immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (B) Representative Chk2 immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (C) Representative γ-H2A.X immunohistochemical micrographs (×400 magnification, scale bar = 25 μm). (D) Statistical results of the percentage of 8-OHdG-positive area in WT and KI mice. (E) Statistical results of the percentage of 8-Chk2-positive area in WT and KI mice. (F) Statistical results of the percentage of γ-H2A.X-positive area in WT and KI mice. (G) Western blot images of 8-OHdG and γ-H2A.X in lung tissues, with β-actin as loading control. (H) Statistical comparison of 8-OHdG and γ-H2A.X protein expression levels. (A-H) Data are from three independent biological replicates (n = 3 WT and 3 KI mice). Adjusted P-value = Original P-value × Number of multiple comparisons (Bonferroni correction) adjusted p = raw p ×5 *p < 0.05, **p < 0.01, ***p < 0.001.
