Fig. 1

A ring plot highlighting the unique sequence region employed in developing a loop-mediated isothermal amplification (LAMP) assay for the specific detection of Xanthomonas axonopodis pv. vasculorum. This circular graphic illustrates multiple genome alignments that include six X. axonopodis pv. vasculorum genomes (three of which were obtained from our lab) and ten other Xanthomonas species that share the same ecological niche or infect the same host, sugarcane. The first three innermost layers of the graphic represent the genome coordinates (in megabase pairs, mbp), the GC content (depicted as a zigzag black line), and the GC skew (shown as a zigzag purple + / green - line) of the reference genome X. axonopodis pv. vasculorum strain NCPPB 796. Subsequent color-coded rings display the BLASTn pairwise comparisons of various X. axonopodis pv. vasculorum strains, including NCPPB 796 (NZ_CP053649), NCPPB 900 (NZ_JPHD00000000), CFBP5823 (MDCD00000000), LMG894, LMG901, and LMG903. These are followed by the position of the unique target region conserved across all X. axonopodis pv. vasculorum strains, which is pointed out and labeled in red. Additionally, the plot includes genomes from other Xanthomonas species, such as X. campestris pv. campestris ATCC 33,913 (NC_003902), X. campestris pv. musacearum NCPPB 4379 (NZ_AKBF00000000), X. citri pv. citri MN12 (NZ_CP008998), X. euvesicatoria pv. alfalfae CFBP3836 (NZ_CP072268), X. oryzae pv. oryzae ICMP3135 (NZ_CP031697), X. oryzae pv. oryzicola GX01 (NZ_CP043403), X. phaseoli pv. dieffenbachiae LMG695 (NZ_CP014347), X. perforans GEV872 (NZ_CP116305), X. vasicola pv. vasculorum Xv1601 (NZ_CP025272), and X. sacchari DJ16 (NZ_CP121698). This image was generated using the BLAST Ring Image Generator (BRIG) v 0.95²⁵.