Fig. 2
From: Genome guided LAMP assay for rapid and reliable detection of Xanthomonas axonopodis pv. vasculorum
The specificity of the loop-mediated isothermal amplification (LAMP) assay, designed for the selective amplification of Xanthomonas axonopodis pv. vasculorum, was validated using 87 bacterial strains, as outlined in the inclusivity and exclusivity panels in Table 2. Identify A as a real-time LAMP assay, detected using a qPCR machine, while B and C are visual and under UV detection methods. (A) Curves surpassing the threshold of 0.2 normal fluorescence indicate a positive result. All strains from the inclusivity panel were positive, and all strains from the exclusivity panel tested negative. (B) SYBR Green I dye was added to each tube, with Xav strains in tubes 1–6 (LMG901, LMG894, LMG897, LMG903, LMG8709, LMG8715) and exclusivity strains in tubes 7–12 (A6252 - X. citri pv. aracearum, A5592 - X. sacchari, A6237 - X. sonti, A6256 - X. phaseoli pv. dieffenbachiae, A1809 - X. euvesicatoria, A4724 - X. campestris pv. campestris). Positive results changed to green, while negative results remained orange. (C) Positive SYBR Green binding was verified by fluorescence emission detection under UV light.
