Table 1 Details of endpoint PCR and loop-mediated isothermal amplification (LAMP) primers designed for specific detection of Xanthomonas axonopodis pv. vasculorum using the unique target gene region.
From: Genome guided LAMP assay for rapid and reliable detection of Xanthomonas axonopodis pv. vasculorum
Primer | Sequence (5′-3′) | Length (nt) | GC% | Assay |
|---|---|---|---|---|
XCV1-FP | CTTGCACACGAAACGCTTCC | 20 | 61 | Endpoint PCR |
XCV1-RP | AACGACATCACCAAGGACGG | 20 | 60 | |
XCV1-FIP | ATGGCCGTATTGCTGATCCACTGCGGCAACGGCTTTTATGC | 41 | 54 | LAMP |
XCV1-BIP | AATGCACGCTCCAACTACGACAGATAGTAGCCGGCCACGT | 40 | 55 | |
XCV1- F3 | CGCCATGTGATTCGCTTCAA | 20 | 55 | |
XCV1-B3 | CCCGTAGCCCAGGTTGTTG | 19 | 58 | |
XCV1-LF | CGCCGATCTGGTGATTG | 17 | 59 | |
XCV1-LB | CTCTCCACGCTGGCCGACAA | 20 | 65 |
