Table 2 List of strains used in the inclusivity and exclusivity for the validation assay of Xanthomonas axonopodis pv. vasculorum specific loop mediated isothermal amplification (LAMP) and endpoint PCR.

From: Genome guided LAMP assay for rapid and reliable detection of Xanthomonas axonopodis pv. vasculorum

Species

Strain ID

Host

Origin

Results

LAMP

PCR

Inclusivity panel

 Xanthomonas axonopodis pv. vasculorum

LMG894

Saccharum officinarum

Australia

Positive

Positive

 X. axonopodis pv. vasculorum

LMG897

S. officinarum

Mauritius

Positive

Positive

 X. axonopodis pv. vasculorum

LMG901

S. officinarum

Mauritius

Positive

Positive

 X. axonopodis pv. vasculorum

LMG903

Thysanolaena maxima

Mauritius

Positive

Positive

 X. axonopodis pv. vasculorum

LMG8709

Roystonea regia

Mauritius

Positive

Positive

 X. axonopodis pv. vasculorum

LMG8715

S. officinarum

Reunion

Positive

Positive

Exclusivity panel

 Stenotrophomonas aracearum sp. nov.

A5588

Anthurium sp.

Hawaii, USA

Negative

Negative

 S. oahuensis sp. nov.

A5586

Anthurium sp.

Hawaii, USA

Negative

Negative

 Stenotrophomonas sp.

A1974

Syngonium sp.

Hawaii, USA

Negative

Negative

 X. arboricola

A6244

Epipremnum sp.

Hawaii, USA

Negative

Negative

 X. arboricola

A1964

Anthurium sp.

Florida, USA

Negative

Negative

 X. arboricola

A2112

Colocasia sp.

Hawaii, USA

Negative

Negative

 X. arboricola

A5600

Colocasia sp.

Hawaii, USA

Negative

Negative

 X. campestris pv. campestris

A4724

Dieffenbachia sp.

Hawaii, USA

Negative

Negative

 X. citri pv. aracearum

A6252

Xanthosoma sp.

Florida, USA

Negative

Negative

 X. citri pv. aracearum

A6232

Syngonium sp.

Hawaii, USA

Negative

Negative

 X. euvesicatoria

A1809

Caladium sp.

Florida, USA

Negative

Negative

 X. euvesicatoria

A1986

Dieffenbachia sp.

Florida, USA

Negative

Negative

 X. euvesicatoria

A1989

Dieffenbachia sp.

Florida, USA

Negative

Negative

 X. euvesicatoria

A1962

Philodendron sp.

Hawaii, USA

Negative

Negative

 X. euvesicatoria

A3651

Polyscias guilfoylei

Hawaii, USA

Negative

Negative

 X. euvesicatoria

A3650

P. guilfoylei

Hawaii, USA

Negative

Negative

 X. hawaiiensis sp. nov.

A2111

Colocasia sp.

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A6241

Aglaonema sp.

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A4718

Anthurium sp.

India

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A4719

Anthurium sp.

India

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A2650

Anthurium sp.

Jamaica

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A5172

Anthurium sp.

Mauritius

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A4723

Anthurium sp.

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A6245

Anthurium sp.

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A6249

Anthurium sp.

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A6256

Anthurium sp.

Hawaii, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

A2681

Aroid

Hawaii, USA

Negative

Negative

 X. phaseoli pv. dieffenbachiae

A2108

Dieffenbachia sp.

Hawaii, USA

Negative

Negative

 X. sacchari

A5592

Anthurium sp.

Hawaii, USA

Negative

Negative

 X. sacchari

A6251

Spathiphyllum sp.

Hawaii, USA

Negative

Negative

 X. sontii

A6237

Aglaonema sp.

Hawaii, USA

Negative

Negative

 X. euvesicatoria

A1978

Philodendron sp.

Florida, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

A6305

Anthurium sp.

Hawaii, USA

Negative

NT

 X. euvesicatoria

A3794

Solanum lycopersicum

Taiwan

Negative

NT

 X. euvesicatoria

A1711

S. lycopersicum

California, USA

Negative

NT

 X. euvesicatoria

A3481

S. lycopersicum

Taiwan

Negative

NT

 X. euvesicatoria

A3614

S. lycopersicum

South America

Negative

NT

 X. euvesicatoria

A1786

Capsicum sp.

Hawaii, USA

Negative

NT

 X. vesicatoria

A3791

S. lycopersicum

New Zealand

Negative

NT

 X. vesicatoria

A1717

S. lycopersicum

California, USA

Negative

NT

 X. euvesicatoria

A4468

S. lycopersicum

Taiwan

Negative

NT

 X. campestris pv. campestris

A1694

S. lycopersicum

California, USA

Negative

NT

 X. euvesicatoria

A6260

S. lycopersicum

Florida, USA

Negative

NT

 Pseudomonas fluorescens

A4583

Anthurium sp.

Hawaii, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

A6234

Anthurium sp.

Hawaii, USA

Negative

NT

 X. vesicatoria

A1696

S. lycopersicum

California, USA

Negative

NT

 Pantoea agglomerans

DP150B

Zea mays

NE, USA

Negative

NT

 Curtobacterium flaccumfaciens pv. flaccumfaciens

DP122B

Z. mays

NE, USA

Negative

NT

 Pseudomonas syringae pv. syringae

A6178

S. lycopersicum

Georgia, USA

Negative

NT

 Klebsiella sp.

A6223

Z. mays

Iowa, USA

Negative

NT

 Microbacterium sp.

A6214

Z. mays

Iowa, USA

Negative

NT

 Pantoea ananatis

DP149

Z. mays

TX, USA

Negative

NT

 P. agglomerans

A6216

Z. mays

TX, USA

Negative

NT

 P. ananatis

A6220

Z. mays

Iowa, USA

Negative

NT

 P. syringae pv. syringae

A3830

Oryza sativa

South Africa

Negative

NT

 Agrobacterium tumefaciens

A2961

Prunus avium

New York, USA

Negative

NT

 Pseudomonas sp.

DP142

Z. mays

IA, USA

Negative

NT

 Pectobacterium brasiliense

PL64

Brassica oleraceae

Hawaii, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

PL36

Anthurium sp.

Hawaii, USA

Negative

NT

 Erwinia amylovora

A1084

Pyrus sp.

Negative

NT

 Clavibacter michiganensis

A1741

S. lycopersicum

California, USA

Negative

NT

 Pectobacterium carotovorum

PL73

Solanum tuberosum

Hawaii, USA

Negative

NT

 Ralstonia solanacearum

A5658

S. lycopersicum

Ontario, Canada

Negative

NT

 Clavibacter sepedonicus

A2041

S. tuberosum

Denmark

Negative

NT

 Rathayibacter tritici

LMG3726

Triticum aesticum

Egypt

Negative

NT

 Dickeya dianthicola

PL24

S. tuberosum

Hawaii, USA

Negative

NT

 D. solani

LMG25993

S. tuberosum

Netherlands

Negative

NT

 D. zeae

PL47

Brassica oleraceae var. sabellica

Hawaii, USA

Negative

NT

 D. fangzhongdai

PL149

C. esculenta

Hawaii, USA

Negative

NT

 Klebsiella aerogene

A3131

unknown

unknown

Negative

NT

 P. ananatis

3

Panicum maximum

Hawaii, USA

Negative

NT

 Pantoea stewartii

13 A

Setaria verticillata

Hawaii, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

A5362

Anthurium sp.

Hawaii, USA

Negative

NT

 P. brasiliense

PL246

Brassica rapa subsp. chinensis

Hawaii, USA

Negative

NT

 D. dadantii

A1042

Philodendron sp.

Hawaii, USA

Negative

NT

 X. phaseoli pv. dieffenbachiae

A5721 (XAD)

Anthurium sp.

Hawaii, USA

Negative

NT

 X. oryzae pv. oryzicola

A3545

O. sativa

Negative

NT

 Ralstonia solanacearum

A5538

Solanum nigrum

Nairobi, Kenya

Negative

NT

 Klebsiella sp.

A223

Vanda sp.

Negative

NT

 X. oryzae pv. oryzicola

A3542

O. sativa

Negative

NT

 Rathayibacter toxicus

WAC3373

Phalaris paradoxa

Western Australia

Negative

NT

Healthy host/soil

 Healthy Rice

O. sativa

Negative

NT

 Healthy sugarcane leaf

Saccharum sp.

Negative

NT

 Soil

 

Negative

NT

  1. Note: most of the DNA used in this study were taken from previous or ongoing studies in the laboratory. NT is not tested.
  2. “–” information not available.