Fig. 4
From: A subpopulation of projections from the parabrachial nucleus to the central amygdala mediates itch
Optogenetic stimulation of serotonin-responsive PBN-CeA projections does not affect anxiety-like behavior. Serotonin- or PBS-TRAPed mice received bilateral intra-PBN injections of AAV-DIO-hChR2(H134R)-EYFP, and optic fibers were implanted above the CeA. Anxiety- and locomotion-related behaviors were assessed using the elevated plus maze (EPM) and open field test (OFT) under light-off and light-on conditions. (A, B) In the EPM test, time spent in the open arms (a measure of thigmotaxis) was recorded for serotonin-TRAPed (A) and PBS-TRAPed (B) mice (n = 10/group). (C, D) Total arm entries (a measure of locomotion) were recorded for serotonin-TRAPed (C) and PBS-TRAPed (D) mice (n = 10/group). (E, F) Percentage of center square entries (another measure of thigmotaxis) was assessed in serotonin-TRAPed (E) and PBS-TRAPed (F) mice (n = 10/group). (G, H) In the OFT, total square entries (a measure of locomotion) were recorded for serotonin-TRAPed (G) and PBS-TRAPed (H) mice (n = 10/group). Data were analyzed using two-way repeated-measures ANOVA followed by Bonferroni’s multiple comparison test. No significant differences were observed (p > 0.05). Error bars represent SEM.
