Fig. 2
Reversibility assay. Infected BeWo cells were exposed to C2 (8 µg/mL), C4 (8 µg/mL), C9 (32 µg/mL), S + P (200 + 8 µg/mL) or only culture medium (M) for 24 h, followed or not by treatment removal for an additional 24 h. The β-galactosidase assay was performed and T. gondii intracellular proliferation was expressed in percentages (% of T. gondii proliferation). Treatment reversibility was deliberate by comparing the conditions of 24 h of treatment with the 24 h after treatment removal. The negative control group (M) was considered as 100% reversible. Data are expressed as means ± SEM from three experiments performed in eight replicates. (*) Comparisons between infected/untreated and infected/treated cells; (#) S + P and chalcone-treated cells; and (&) between infected/treated cells 24 h after treatment with the infected/treated cells 24 h after treatment removal. Significant differences were analyzed using (*;#) One-Way ANOVA with Dunnett’s multiple comparison post-test and (&) Unpaired Student´s t-test (two-tailed). Differences were considered as statistically significant when P < 0.05.
