Fig. 1

Generation and characterization of brain organoids (a) Schematic diagram of the brain organoid protocol. (b) Light microscope images of brain organoids from 35 d – 63 d. Scale bars, 200 μm. (c) Quantification of brain organoid size from 35 d – 63 d using diameter and surface area measurements. Values represent mean ± IQR (n = 10). (d) Immunostaining for DAPI (blue), SOX2 (red), TBR2 (purple), and CTIP2 (green) of brain organoids on 63 d. Scale bars, 50 μm. ∗∗∗p < 0.0001.