Fig. 2
Effect of haloperidol on human brain organoids treated with haloperidol (a) Schematic diagram of haloperidol (HAL) exposure time on brain organoids (upper). Light microscope images of brain organoids treated with HAL from 35 d – 63 d. Scale bars, 200 μm. (b) Quantification of brain organoid size with or without HAL treatment from 35 d – 63 d using surface area and diameter measurements. Values represent mean ± IQR (n ≥ 6). (c) Immunostaining for DAPI (blue), SOX2 (red) and TUJ1 (green) of brain organoids treated with HAL at 63 d. Scale bars, 50 μm. (d) Quantification of the thickness of VZ-like regions and neuronal layers of organoids treated with HAL on 63 d. Values represent mean ± IQR (n = 5 cortical structures from three organoids). ∗p < 0.05, ∗∗p < 0.005 ∗∗∗p < 0.0001.
