Fig. 3 | Scientific Reports

Fig. 3

From: Chronic haloperidol exposure impairs neurodevelopment via Notch1 signaling in human stem cell-derived brain organoids

Fig. 3

Effect of haloperidol on human brain organoids treated with haloperidol and sodium propionate (a) Light microscope images of brain organoids treated with haloperidol (HAL) and sodium propionate (PRO) from 35 d – 63 d. Scale bars, 200 Î¼m. (b) Quantification for brain organoid size after HAL and PRO treatment from 35 d – 63 d using surface area and diameter measurements. Values represent mean ± IQR (n ≥ 6). (c) Immunostaining for DAPI (blue), SOX2 (red) and TUJ1 (green) of brain organoids treated with HAL and PRO (300µM) on 63 d. Scale bars, 50 Î¼m. (d) Quantification of the thickness of VZ-like regions and neuronal layers of organoids treated with HAL and PRO (300µM) on 63 d. Values represent mean ± IQR (n = 5 cortical structures from three organoids). ns; non-significant. ∗p < 0.05.

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