Fig. 5
Periplocin regulates DR4 expression in thyroid cancer cells. (A and B) Western blotting was used to detect DR4 expression in BCPAP and TPC-1 cells treated with periplocin for 24 h. ImageJ analysis of gray values was normalized to internal controls. (C and D) A Western blot (WB) test was done to check for DR4 levels in BCPAP and TPC-1 cells after they were treated with siRNA. DR4 expression in BCPAP and TPC-1 cells transfected with siRNA was quantified using ImageJ (the greyscale values were normalised to β-actin) and presented as a histogram. *P < 0.05 vs. control; **P < 0.01 vs. control; ***P < 0.001 vs. control. DAMPs: Damage-Associated Molecular Patterns; DR4: Death Receptor 4; MLKL, Mixed Lineage Kinase Domain-Like Protein; RIP3: Receptor-Interacting Protein Kinase 3.
