Fig. 7

Effects of periplocin and its mechanism in BALB/c nude mice. (A and B) Representative images of mice and tumors in control and periplocin-treated groups. (C) Tumor weights. (D) Immunohistochemical detection of PCNA, p-MLKL, and p-RIP3 expression; IOD calculated using ImageJ. Data expressed as mean ± standard deviation (SD). (E) Mean tumor volume over time. (F) PI-positive rate detected using Hoechst 33,342/PI staining. ***P < 0.001. IOD: Integrated Optical Density; MLKL: Mixed Lineage Kinase Domain-Like Protein; PCNA: Proliferating Cell Nuclear Antigen; PI: Propidium Iodide; RIP3: Receptor-Interacting Protein Kinase 3.