Fig. 4
From: Fisetin limits Chikungunya virus-induced apoptosis hallmarks in hepatocellular carcinoma cells
Cleaved PARP levels at 48 hpi, assessed via immunoblot, showed high apoptosis in CHIKV-infected cells. Z-VAD-FMK treatments reduced apoptosis, with fisetin showing the most significant reduction. Also shown in this figure is the relative quantification of the proteins analyzed using ImageJ. Cut PVDF membrane sections were used for antibody hybridization to optimize reagent consumption while maintaining experimental validity. The uncropped/original version can be visualized in the Supplementary Figure S1.
