Fig. 5
From: MAN2A2-related glycosylation defects in autism and cognitive delay
UHPLC-ESI MS analysis of MAN2A2 diagnostic species in patient serum sample compared to two age-matched controls. (a) Overlaid Extracted ion chromatograms (EICs) of RFMS labelled oligomannose serum N-glycans showing in the patient (red line) the increase of all the species from Man 5 to Man 9 with respect to controls (blue and green line). (b) Overlaid EICs of RFMS-labelled hybrid-type N-glycans with their major assignments, showing in the patient the accumulation of some specific disease-associated structures (as highlighted by red circles), especially the penta-mannosylated ones with shorter elongations at α1-3 mannose branch. Each chromatographic profile is normalized with respect to the dominant disialo-biantennary structure. Glycan symbols: N-acetylglucosamine, blue square; mannose, green circle; galactose: yellow circle; sialic acid: purple diamond (+ 45°: α2-6 linked; -45°: α2-3 linked); fucose: red triangle.
