Table 2 Comparative hydrogen bonding analysis of wild-type (WT) and Arg560Gln mutant (MUT) MAN2A2 AlphaFold3 predictions in the alpha mannosidase middle domain (PF09261; IPR015341).
From: MAN2A2-related glycosylation defects in autism and cognitive delay
Donor residue | Donor atom | Acceptor residue | Acceptor atom | Distance (Å) | Conservation status |
|---|---|---|---|---|---|
Arg540 | NH1 | Ser1069 | O | 3.23 (± 0.06) | WT-specific (absent in Model 4) |
Leu555 | N | Asp552 | O | 2.97 (± 0.02) | MUT-specific (absent in Model 3) |
Arg560 | N | Leu556 | O | 2.87 (± 0.01) | WT-specific (all models) |
Arg560 | NE | Glu529 | OE1 | 2.51 (± 0.01) | WT-specific (all models) |
Arg560 | NH1 | Thr1059 | O | 3.43 (± 0.03) | WT-specific (all models) |
Arg560 | NH2 | Glu529 | OE1 | 2.78 (± 0.08) | WT-specific (all models) |
Arg560 | NH2 | Glu529 | OE2 | 2.73 (± 0.01) | WT-specific (absent in Model 2) |
Arg560 | NH2 | Thr1059 | O | 2.94 (± 0.06) | WT-specific (all models) |
Gln560 | N | Leu556 | O | 2.85 (± 0.01) | MUT-specific (all models) |
Gln560 | NE2 | Glu529 | OE1 | 2.22 (± 0.05) | MUT-specific (all models) |
Gly564 | N | Arg560 | O | 2.82 (± 0.01) | WT-specific (all models) |
Gly564 | N | Gln560 | O | 2.82 (± 0.01) | MUT-specific (all models) |
His569 | N | Met252 | O | 3.41 (± 0.01) | MUT-specific (Models 0,3 only) |
Glu578 | N | Gln974 | OE1 | 3.52 (± 0) | WT-specific (Models 0,2 only) |
Arg590 | NH2 | Glu558 | OE2 | 3.25 (± 0.2) | WT-specific (Models 1,2 only) |
Gln597 | NE2 | His601 | NE2 | 2.38 | WT-specific (Model 3 only) |
