Fig. 4
From: PSAT1 inhibits ferroptosis in osteosarcoma cells by activating the Xct/GPX4 signaling axis
PSAT1 silencing inhibited the proliferation, migration and invasion of osteosarcoma cells. (A) RT-qPCR was used to detect the expression of PSAT1 mRNA in 143B and MG-63 cells. (B) Western blot was used to detect the expression of PSAT1 protein in 143B and MG-63 cells. (C) CCK8 assay was used to detect the effect of PSAT1 on the proliferation of 143B and MG-63 cells. (D) Cell clone formation method for 143B and MG-63 cell proliferation ability. (E,F) The effect of PSAT1 on the migration and invasion ability of 143B and MG-63 cells was detected by Transwell chamber assay (× 20 times). (G) The effect of PSAT1 on the cell cycle of 143B and MG-63 cells was detected by flow cytometry. sh-PSAT1: silencing PSAT1 group; sh-NC: control group. *p < 0.05; **p < 0.01; ***p < 0.001.
