Fig. 6
From: PSAT1 inhibits ferroptosis in osteosarcoma cells by activating the Xct/GPX4 signaling axis
Silencing PSAT1 may promote ferroptosis in OS cells. (A) The ferroptosis-related proteins in each group were determined by Western blot. (B) After silencing PSAT1 or adding ferrostatin-1, the relative cell viability of each group was measured by CCK8 assay. (C) After silencing PSAT1 and adding ferrostatin-1, the relative contents of MDA, ferrous ion and GSH in each group were measured by kits. (D) Flow cytometry was used to measure the relative content of ROS in each group after silencing PSAT1 or adding ferrostatin-1. (E) The accumulation of lipid peroxidation was measured by C11-BODIPY staining after silencing PSAT1 or adding ferrostatin-1. sh-PSAT1: silencing PSAT1 group; sh-NC: control group, fer-1 ferrostatin-1. *p < 0.05; **p < 0.01; ***p < 0.001.
