Table 1 PCR primers corresponding to differentially expressed genes for ferroptosis in osteosarcoma.
From: PSAT1 inhibits ferroptosis in osteosarcoma cells by activating the Xct/GPX4 signaling axis
Genes | Primer sequence (5'–3') | Length of fragment (bp) |
|---|---|---|
Primer sequences of PCR | ||
 CYBB | qPCR Forward: CCACGGCTCTGCCCAGGTTA qPCR Reverse: TGGCTTAGGAGCTTGAAGTTGACC | 287 |
 ALOX5 | qPCR Forward: GTCCACCAGACCATCACCC qPCR Reverse: CGTTGGCCTTGTCAAAGAG | 196 |
 CBS | qPCR Forward: 5-GTACCGCAACGCCAGCAACC-3 qPCR Reverse: 5-ATCCAGGACACTTCTCCTTCAGCT-3 | 158 |
 PSAT1 | qPCR Forward: TACCCGATGTCAAGGGAGCAG qPCR Reverse: TTGTATTCCAGGACCGAGGG | 199 |
 HBA1 | qPCR Forward: CCACGGCTCTGCCCAGGTTA qPCR Reverse: TGGCTTAGGAGCTTGAAGTTGACC | 254 |
 ASNS | qPCR Forward: GTAGTTGACCCGCTGTTTGG qPCR Reverse: ACCCAGGAACACTTTCTTATT | 255 |
 TRIB3 | qPCR Forward: GAGATACTCAGCTCACGGGCCTCA qPCR Reverse: AAGGGCATCGGGTCCTGTCG | 281 |
 GAPDH | qPCR Forward: GATTCCACCCATGGCAAATTC qPCR Reverse: CTGGAAGATGGTGATGGGATT | 87 |
