Fig. 7 | Scientific Reports

Fig. 7

From: Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro

Fig. 7

Differential staining of the inner cell mass (ICM) and trophectoderm (TE), and TUNEL assay of TLR2 agonist-treated bovine Day 7 blastocysts. (a) Representative images of Hoechst 33,342 (Total DNA, blue), PI (TE cell, red), and transferase-mediated dUTP nick end labeling (TUNEL, apoptotic nuclei, green) stained Day 7 in vitro–produced bovine embryos. The merged image was TUNEL, PI and Total DNA. Scale bar = 50 μm. (b) The effects of TLR2 activation on the number of ICM, TE, and apoptosis-related indexes. (c) The effect of TLR2 activation on the number of ICM, TE, and apoptosis-related indexes at different stages: BL, ExB, and HB. The asterisk denotes a significant variance between the ‘control (n = 66)’ and ‘TLR2 agonist (n = 86)’ groups. Data show mean ± S.E.M (n = 5), *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group.

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