Fig. 2
From: Neprilysin 2 catalyses the degradation of natriuretic peptides despite sacubitrilat Inhibition
Analysis of ANP and BNP cleavage by NEP and NEP2 in vitro. The NPs were incubated with NEP, NEP2 or without enzymes in the presence or absence of inhibitors: phosphoramidon (probes NEP + Ph. and NEP2 + Ph.) and sacubitrilat (NEP + Sac. and NEP2 + Sac.). (A and C). ANP immunoreactivity was assessed using intact-ring ANP-s-IA. BNP immunoreactivity assessed with intact-ring BNP s-IA. Error bars represent SD. In all cases, concentrations are expressed relative to the initial concentration of the peptide in the absence of the enzyme at each time point. Asterisks mark points where concentration difference from no enzyme probe was statistically significant (P < 0.05).
