Fig. 3
From: Neprilysin 2 catalyses the degradation of natriuretic peptides despite sacubitrilat Inhibition
Analysis of proBNP cleavage by NEP2. ProBNP was incubated with NEP, NEP2 or without enzymes in the presence or absence of phosphoramidon (probes NEP + Ph. and NEP2 + Ph.). (A) Immunoreactivity of proBNP, assessed with intact-ring BNP s-IA, shown as the percentage of peptide immunoreactivity in the enzyme-free sample. Error bars represent SD. In all cases, concentrations are expressed relative to the initial concentration of the peptide in the absence of the enzyme at each time point. Asterisks mark points where concentration difference from no enzyme probe was statistically significant (P < 0.005). (B) MS analysis of cleavage samples before (left panel) and after 2 h incubation (right panel).
