Fig. 2 | Scientific Reports

Fig. 2

From: Lycium barbarum glycopeptide mitigates retinal ischemia-reperfusion injury through its anti-inflammatory, anti- senescence, and anti-apoptosis properties

Fig. 2

Anti-inflammatory effects of LbGp. (A) IF demonstrates LbGp-mediated modulation of IL-6 expression in the retinas of RIRI mice, scale = 50 μm. Dual fluorescence labeling reveals IL-6 (red) and nuclear DNA (blue) distribution patterns in retinal tissues. (B) IF demonstrates LbGp-mediated modulation of IL-1β expression in the retinas of RIRI mice, scale = 50 μm. Dual fluorescence labeling reveals IL-1β (red) and nuclear DNA (blue) distribution patterns in retinal tissues. (C) Quantitative analysis of IL-6 and IL-1β fluorescence intensity in mice. Values represent mean ± SD from triplicate experiments: **p < 0.01 vs. Sham group; #p < 0.05 vs. RIRI group. (D) IF demonstrates LbGp-mediated modulation of IL-6 and IL-1β levels in OGD/R cells, scale = 20 μm. Triple fluorescent labeling reveals the distribution pattern of IL-6 (red), IL-1 (green), and nuclear DNA (blue) in cells. (E) Quantitative analysis of cellular IL-6 and IL-1β fluorescence intensity. Values represent mean ± SD from triplicate experiments: **p < 0.01 vs. Ctrl group, #p < 0.05 vs. OGD/R group. (F) ELISA showing levels of IL-6, IL-1β and TNF-α in peripheral blood of mice. Data are expressed as mean ± SD (n = 6), **p < 0.01 vs. Sham group, #p < 0.05 vs. RIRI group. (G) ELISA showed the levels of IL-6, IL-1β and TNF-α in cell supernatants. Data are expressed as mean ± SD (n = 6), **p < 0.01 vs. Ctrl group, ##p < 0.01 vs. OGD/R group.

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